中国实用口腔科杂志

中国实用口腔科杂志

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口腔鳞癌组织中人β-防御素-1、-2、-3表达水平及其与临床TNM分期关系研究

房栗1，2，李国阳1，刘婷1，黄晓峰1，王翔1，王文梅1   

  1. 1.南京大学医学院附属口腔医院·南京市口腔医院，江苏  南京  210008；2.苏州口腔医院，江苏  苏州  215008
  • 出版日期:2018-03-15 发布日期:2018-04-17
  • 基金资助:

    国家自然科学基金（81570978）；江苏省临床医学科技专项基金（BL2014018）；江苏省青年医学人才项目（QNRC2016118）；南京市卫生局医学科技发展重点项目（ZKX17033）

  • Online:2018-03-15 Published:2018-04-17

摘要：

目的    初探口腔鳞癌（oral squamous cell carcinoma，OSCC）组织中人β-防御素（human β-defensin，hBD ）-1、hBD-2、hBD-3的表达水平及其与临床TNM分期的关系。方法    采用免疫组化染色法，检测60例OSCC组织中hBD-1、hBD-2和hBD-3的表达，评价其染色分级及表达水平，统计分析hBD-1、hBD-2和hBD-3免疫组化染色分级之间的相关性，并进一步分析OSCC组织中hBD-1、hBD-2和hBD-3染色分级与临床TNM分期之间的相关性。结果    OSCC组织中hBD-1与hBD-3 的染色分级之间呈显著正相关（P < 0.01），hBD-3的染色分级与临床TNM分期之间呈显著负相关（P < 0.05）。结论    OSCC组织中hBDs的表达水平之间存在一定的联系，互为影响；hBD-3表达水平与OSCC临床TNM分期之间存在相关性，随着TNM分期的增加而降低。

关键词: 口腔鳞状细胞癌, 人&beta, 防御素, TNM分期, 相关性分析

Abstract:

Objective    To investigate the expression level of human β-defensin -1，-2 and -3 in OSCC and its relationship with clinical TNM staging. Methods    Immunohistochemical staining was adopted to test the expression of hBDs in OSCC tissues. The stain grades were evaluated to represent the expression levels. The correlations between the staining grades of hBD-1，-2 and -3 were statistically analyzed and the correlations between the staining grades of hBDs and the TNM stages of OSCC were further analyzed. Results    There was a significantly positive correlation between the staining grade of hBD-1 and that of hBD-3 （P < 0.01）. There was a significantly negative correlation between the staining grades of hBD-3 and the TNM stages （P < 0.05）. Conclusion    The expression levels of human β-defensin -1，-2 and -3 in OSCC influence each other，suggesting that the expression livel of hBD-3 is related to the clinical staging of OSCC. The expression level of hBD-3 in OSCC could decrease along with the increasing TNM stage.

Key words: oral squamous cell carcinoma；human &beta, -defensin；TNM stage；correlation analysis