中国实用口腔科杂志

中国实用口腔科杂志

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牙本质基质蛋白-1参与下颌功能前伸过程中髁突软骨增殖的实验研究

汪浩然，左艳萍，闫宝勇，马素伟，李爽   

  1. 河北医科大学第一医院，河北  石家庄  050030（第一作者现工作于江苏省沭阳县中医院，江苏  宿迁  223699）
  • 出版日期:2017-09-15 发布日期:2017-11-22
  • 基金资助:

    河北省卫计委医学科学研究课题计划（20160712）

  • Online:2017-09-15 Published:2017-11-22

摘要：

目的    探讨牙本质基质蛋白-1（dentin matrix protein，DMP-1）对功能矫治前伸下颌过程中髁突骨软骨增殖的作用，为临床骨性Ⅱ类错牙合矫形提供实验依据。方法    选用4周龄健康雄性Sprague-Dawley（SD）大鼠70只，随机等量分为实验组和对照组，实验组大鼠全天佩戴下颌前伸斜面导板，对照组不做任何处理。于实验第1、3、7、14、21、28和35 天每组分别处死大鼠5只，取双侧髁突，采用免疫组织化学方法及细胞图像分析系统检测DMP-1的表达变化。结果    实验组大鼠髁突软骨中DMP-1表达于第3天开始增强，至第14天达到最高峰，强度-色调-饱和度（IHS）值（85.67±4.51）与对照组（10.67±1.53）相比差异有统计学意义（P＜0.05）。结论    DMP-1参与大鼠下颌前伸过程髁突软骨适应性改建，促进髁突软骨增殖和软骨内成骨。

关键词: 下颌功能前伸, 髁突软骨, 骨改建, DMP-1

Abstract:

Objective    Objective To investigate the changes in the expression of DMP-1 in the condylar of a young rat during functional mandibular protrusion. This work also explores the function of DMP-1 in the cartilage proliferation，which provides experimental evidence for the clinical orthopedic treatment for bone type II malocclusion. Methods     Totally 70 healthy male Sprague-Dawleys （SD） rats at the age of 4 weeks were included in the study. The rats were divided randomly and equally into experimental group and control group. The mandibular protrusion bite plate appliances that were fitted to the animals in the experimental group were worn all day. No operation was performed in the control group. Five rats were executed in each group after 1，3，7，14，21，28 and 35d. Detect the expression and variation of DMP-1 by immunohistochemical method and cell image analysis system. Results    On the 3rd day，the expression of DMP-1 in condylar cartilage was obviously enhanced in the experimental group. which reached to the highest level on the 14th day，IHS= 85.67±4.51, which had significant difference （P < 0.05） compared with control group，IHS=10.67±1.53. Conclusion    DMP-1 participates in condylar cartilage adaptive remodeling during the process of mandibular protrusion of rats，and promotes condylar cartilage proliferation and endochondral ossification.

Key words: mandibular functional protrusion, condylar cartilage, bone remodeling, DMP-1