中国实用口腔科杂志

中国实用口腔科杂志

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牙乳头细胞对大鼠牙髓干细胞增殖的影响及机制研究

张忠提，李琳   

  1. 中国医科大学口腔医学院干诊科，辽宁  沈阳  110002
  • 出版日期:2017-07-15 发布日期:2017-07-25
  • 基金资助:

    辽宁省自然科学基金（2015020558）；辽宁省临床能力建设项目（LNCCC-D17-2015）

  • Online:2017-07-15 Published:2017-07-25

摘要：

目的    探讨大鼠牙乳头细胞在体外与牙髓干细胞（dental pulp stem cells，DPSCs）分层共培养对DPSCs增殖的影响及相关机制。方法    建立DPSCs和牙乳头细胞的共培养体系，观察细胞矿化结节形成情况，并利用流式细胞术检测DPSCs的细胞周期；给予Notch信号通路抑制剂异硫氰酸苯己酯（PHI）干预DPSCs，检测DPSCs中Notch1、Jagged1 mRNA和蛋白的表达变化。结果    分层共培养14 d后，DPSCs中可观察到明显的矿化结节。流式细胞术结果显示共培养可促进DPSCs的增殖。DPSCs和牙乳头细胞的分层共培养下，DPSCs中Notch1、Jagged1 mRNA和蛋白表达较对照组（单纯DPSCs培养）显著增强。结论    牙乳头细胞与DPSCs共培养可能通过激活Notch信号通路，促进DPSCs的矿化与增殖。

关键词: 牙乳头细胞, 牙髓干细胞, 细胞增殖

Abstract:

Objective    To investigate effects of dental papillae cells on the proliferation of dental pulp stem cells（DPSCs） and its mechanism. Methods    DPSCs and dental papillae cells were cultured，the co-cultured systems of DPSCs and dental papillae cells were established. The intracellular mineralization nodes were observed. The cell cycle of DPSCs was detected by flow cytometry. Finally，when the inhibitor of Notch，PHI，was administrated，the expression of Notch1，Jagged1 mRNA and protein was examined. Results    The obvious mineralization nodules were observed in DPSCs after 14 d of co-culture. Flow cytometry results showed that stratified co-culture promoted the proliferation of DPSCs. RT-PCR and western blot results showed that the expression of Notch1，Jagged1 mRNA and protein in the co-culture group was significantly increased compared with control group. Conclusion    Stratified co-culture of DPSCs and dental papillae cells might promote the mineralization and proliferation by activating Notch signal pathway.

Key words: dental papillae cell, dental pulp stem cell, cell proliferation