Study of the effect of NF-κBp65 protein and ENA-78 in the onset of endometriosis.
HAO Min，HOU Yong-li.
Chinese Journal of Practical Gynecology and Obstetrics ›› 2015, Vol. 31 ›› Issue (7) : 640-644.
Study of the effect of NF-κBp65 protein and ENA-78 in the onset of endometriosis.
Abstract： Objective To detect the effect of κBp65 and ENA-78 in the onset of endometriosis. Methods From October 2008 to February 2009 in the Second Hospital of Shanxi Medical University,25 cases undergoing operation resection of ovarian endometriosis cyst were chosen,ectopic and eutopic endometrium tissue was taken,and they were divided into ectopic and eutopic group;select the same normal endometrium of 22 patients as the control group. The eutopic endometrium, ectopic endometrium and thenormal endometrium cells, which were in third passage，were interfered by IL-1β and IL-1β adding PDTC with different concentration. Use ELISA to detect the concentrations of ENA-78 of those three groups，explore the effect of IL-1β and IL-1β adding PDTC on the concentration of ENA-78 of each group.Use immunohistochemistry to detect the activating degree of NF-κBp65 of the endometrial cells in the three groups，and to detect the change of NF-κBp65’s activating degrees after intervention of IL-1β and IL-1β adding PDTC. Results The eutopic endometrium and the ectopic endometrium concentration of the ENA-78 in the cells culture media supernatant was remarkably higher than the normal endometrium.There was a remarkable difference（P<0.01）.The ectopic endometrium concentration of the ENA-78 in the cells culture media supernatant was remarkably higher than the eutopic endometrium.There was a remarkable difference（P<0.01）.After the intervention，the concentration of the ENA-78 in the normal endometrial cells culture media supernatant had no remarkable change.There was no remarkable difference（P>0.05）.In the eutopic endometrium and the ectopic endometrium cells culture media supernatant，the concentration of the ENA-78 in the group intervened with IL-1β was the highest.It was remarkably higher than the control group and the group intervened by IL-1β+PDTC.There was a remarkable difference（P<0.01）. In three groups，the NF-κBp65’s activating degrees of eutopic and the ectopic endometrium were remarkably higher than the normal endometrium.The difference was statistically significant （P<0.05）；for the NF-κBp65’s activating degrees in the eutopic endometrium and the ectopic endometrium cells，the IL-1β group was the highest，which was remarkably higher than the controlled group and the group intervened by IL-1β+PDTC. The difference was statistically significant）（P<0.05）. Conclusion IL-1β can induce the activation of NF-κBp65 on eutopic and ectopic endometrium cells.It can strengthen the expression of ENA-78，while PDTC inhibits this effect.It suggests that as a key controlling agent，NF-κBp65 may play a “penstock” role in the onset of EMs，and it may have a controlling effect on ENA-78 and other cytokines.
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(2015-01-15收稿 2015-03-26修回)
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