Study of the three-dimensional reconstruction model of the nerve and vascular in uterosacral ligament from tissue serial sections.
CHEN Chun-lin*，WANG Jun*，LIU Ping*，WANG Li-ling*，DUAN Hui*，LI Wei-li*，ZHANG Yan，WANG Li，HUANG Zhi-xia*，TANG Lei，LI Jian-yi，ZHONG Shi-zhen
Chinese Journal of Practical Gynecology and Obstetrics ›› 2015, Vol. 31 ›› Issue (6) : 530-534.
Study of the three-dimensional reconstruction model of the nerve and vascular in uterosacral ligament from tissue serial sections.
Abstract： Objective To study the three-dimensional reconstruction model of nerve vascular tissue for uterosacral ligament.Methods A fresh uterosacral ligament specimen was obtained from a stage ⅠB1 cervical cancer patient who underwent abdominal radical hysterectomy.The direction of the uterosacral ligament was fixed with India ink.The processed uterosacral ligament was then cut coronally at a 5 um thickness，every 5 serial layers as a group.Each slice was stained with HE，TH，VIP，D2-40 and artery fibers.All serial sections were given image acquisition，integration，and its registration.Input images into Mimics software，select the appropriate threshold after positioning and interpolation processing，and then reconstruct the three-dimensional digital model of nerve vascular tissue in uterosacral ligament.Results We successfully reconstructed three-dimensional digital model of the nerve and vessel in the uterosacral ligament.
uterosacral ligament / SNSRH / tissue serial sections / three-dimensional reconstruction
［1］ 刘萍，黄蕾，陈春林，等.骶子宫韧带精细显微组织结构定性研究［J］.中国实用妇科与产科杂志，2012，28（9）:691-694.
［2］ 陈春林，王黎，刘萍，等.基于连续切片子宫主韧带内神经脉管数字化三维重建定位材料的探讨［J］.中国数字医学杂志，2014，9（3）: 56-59.
［3］ Butler-Manuel SA，Buttery LD，Polak JM，et al.Autonomic nerve trauma at radical hysterectomy: the nerve content and subtypes within the superficial and deep uterosacral ligaments［J］.Reprod Sci，2008，15 （1）:91-94.
［4］ Hounnou GM，Uhl JF，Plaisant O，et al.Morphometry by computerized three-dimensional reconstruction of the hypogastric plexus of a human fetus［J］.Surg Radiol Anat，2003，25（1）: 21-31.
［5］ Bertrand MM，Alsaid B，Droupy S，et al.Prudhomme. Optimal plane for nerve sparing total mesorectal excision，immunohistological study and 3D reconstruction: an embryological study［J］.Original Article，2013,10(16):1522-1528.
［6］ Fujii S，Takakura K，Matsumura N，et al.Anatomic identification and functional outcomes of the nerve sparing Okabayashi radical hysterectomy［J］.Gynecol Oncol，2007，107（1）: 14-13.
［7］ Katahira A，Niikura H， Ito K，et al.Vesicouterine ligament contains abundant autonomic nerve ganglion cells: the distribution in histology concerning nerve-sparing radical hysterectomy［J］.Int J Gynecol Cancer，2008，18（1）: 193-198.
［8］ Chen CL，Guo HX，Yu YH，et al.The measurement of vesical detrusor electromyographic activity during nerve-sparing radical hysterectomy［J］.Reprod Sci，2010，17（12）: 1144-1152.
（2015-02-01收稿 2015-03-21修回）
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