中国实用妇科与产科杂志

中国实用妇科与产科杂志

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蛋白激酶Cα、β及Rho激酶对宫缩乏力性产后出血产妇子宫平滑肌收缩功能影响研究

颜建英1，周志梅2，廖秋萍1，陈文祯1，罗美瑜1   

  1. 作者单位：1.福建医科大学教学医院 福建省妇幼保健院妇产科，福建 福州350001；2.福建医科大学研究生教育学院，福建 福州350004（现在福建医科大学附属第二医院妇产科工作）
  • 出版日期:2015-03-02 发布日期:2015-04-01
  • 基金资助:

    福建省科技计划重点项目（2009Y0008）；福建省卫生厅医学创新课题（2009-CXB-32）

Effects of PKCα，PKCβ and Rho kinase on human uterine contractility in atonic postpartum hemorrhage.

YAN Jian-ying**，ZHOU Zhi-mei，LIAO Qiu-ping**，CHEN Wen-zhen**，LUO Mei-yu*..   

  1. *Department of Gynaecology and Obstetric，Fujian Medical University Teaching Hospital，Fujian Maternity and Child Health Hospital，Fuzhou 350001，China
  • Online:2015-03-02 Published:2015-04-01

摘要：

目的 探讨蛋白激酶C （PKC）α和PKCβ特异性抑制剂Go6976、PKCβ特异性抑制剂LY333531和Rho激酶特异性抑制剂Y-27632对宫缩乏力性产后出血产妇子宫平滑肌收缩的抑制作用。方法 2010年11月至2011年12月于福建省妇幼保健院选择剖宫产分娩的初产妇60例，分宫缩乏力性产后出血组（病例组，n=30）和宫缩良好无产后出血组（对照组，n=30）。采用肌条等长张力测定方法检测两组产妇子宫下段平滑肌分别在Go6976、LY333531和Y-27632作用下自发性收缩功能变化及病例组在LY333531、Y-27632作用下梯度浓度缩宫素诱导的收缩潜能变化。

关键词: 产后出血, 宫缩乏力, 蛋白激酶C, Rho激酶, 缩宫素

Abstract:

Abstract： Objective To investigate the effects of Go6976（PKCα/β inhibitor），LY333531（PKCβ inhibitor）and Y-27632（Rho kinase inhibitor）on human uterine contractility in atonic postpartum hemorrhage（PPH）. Methods Totally 30 women with atonic PPH as study group and 30 normal pregnant women as control group were included in this study. The myometrial strips from atonic PPH women were adopted to investigate their inhibitory effects by adding Go6976，LY333531 and Y-27632 with isometric tension recording；meanwhile，the effects of LY333531 and Y-27632 on contraction induced by oxytocin were observed following atonic PPH，respectively.Results In study group，the inhibitory effects of Go6976，LY333531 and Y-27632 on amplitude and activity of spontaneous oscillatory contractility were significantly lower than in control group（P<0.05）. There was no significance in frequency between two groups（P>0.05）.LY333531 and Y-27632 further decreased myometrial strips contraction potentiality induced by oxytocin significantly after PPH（P<0.05）. Conclusions PKCα，PKCβ and Rho kinase may be involved in the regulation of myometrial strips contraction，and play an important role in the pathogenesis of atonic PPH.

Key words: postpartum hemorrhage, uterine atony, protein kinase C, Rho-kinase, oxytocin

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