中国实用妇科与产科杂志

• 论著 • 上一篇    下一篇

BMP-2对人卵巢浆液性囊腺癌HO8910细胞浸润能力的影响及机制研究

马颖冷洪锐张淑兰   

  1. 作者单位:中国医科大学附属盛京医院妇产科,辽宁 沈阳110004
  • 出版日期:2014-08-02 发布日期:2014-07-23

Bone morphogenetic protein-2 on the invasion of HO8910 ovarian cancer cells.

MA Ying , LENG Hong-rui, ZHANG Shu-lan.   

  1. Department of Obstetrics and Gynecology, Sheng Jing Hospital of China Medical University,Shenyang 110004,China
  • Online:2014-08-02 Published:2014-07-23

摘要:

目的 检测骨形态发生蛋白-2(BMP-2)对人卵巢浆液性囊腺癌HO8910细胞的浸润能力有无影响,并探讨其可能的影响机制。方法 2008至2009年于中国医科大学盛京医院应用细胞黏附实验、cross-river实验和matrigel-transwell实验观察BMP-2对HO8910细胞浸润能力的影响,并检测BMP-2作用后HO8910细胞中MMP-2、TIMP-2的变化。结果 BMP-2作用后HO8910细胞黏附能力增强,cross-river时间延长,穿过matrigel-transwell侵袭模型的细胞数量减少,且MMP-2表达较前减弱,TIMP-2表达较前增强。结论 BMP-2抑制HO8910卵巢癌细胞浸润能力。

关键词: 卵巢癌, 骨形态发生蛋白-2, 浸润, MMP-2, TIMP-2

Abstract:

Abstract: Objective To verify the influence of bone morphogenetic protein-2 (BMP-2) on the invasion capacity of human ovarian serous cystadenoma cell HO8910, and to determine the possible mechanism.Methods Cell adhesion experiment, cross-river experiment and matrigel-transwell assay were performed to observe the influence of BMP-2 on the invasion ability of HO8910 cells. The changes of MMP-2 and TIMP-2 in HO8910 cells following BMP-2 intervention were also detected in Sheng Jing Hospital of China Medical University between 2008 and 2009.

Key words: ovarian cancer, BMP-2, invasion, MMP-2, TIMP-2

中图分类号: