子宫平滑肌大电导钙激活钾通道α、β 亚基表达与产后出血的关系

摘要/Abstract

摘要：

目的探讨产妇子宫平滑肌大电导钙激活钾通道（BKCa）α、β亚基表达变化在宫缩乏力性产后出血发病中的作用。方法 2009年10月至2011年2月福建省妇幼保健院产科，采用肌条等长张力实验测定30例宫缩乏力性产后出血产妇（病例组）和30例宫缩正常无产后出血产妇（对照组）子宫平滑肌收缩频率、幅度及收缩活动力；应用蛋白印迹法（Western blot）测定两组产妇子宫平滑肌BKCa通道α、β亚基蛋白表达水平；应用实时荧光定量聚合酶链反应（real-time RT-PCR）测定两组产妇子宫平滑肌BKCa通道α、β亚基mRNA表达水平。结果（1）病例组子宫平滑肌自发宫缩收缩频率、幅度、收缩活动力均低于对照组，分别比较差异均有统计学意义（P<0.05）。（2）病例组子宫平滑肌BKCa通道α、β亚基蛋白表达水平及其mRNA表达水平均高于对照组，分别比较，差异均有统计学意义（P<0.05）。（3）两组产妇子宫平滑肌收缩活动力与BKCa通道α、β亚基蛋白表达水平及BKCa通道α、β亚基mRNA表达水平均呈明显负相关（病例组：r分别为-0.401、－0.727及－0.723、－0.895；对照组：r分别为－0.541、－0.378及－0.703、－0.625；[WTBX]P[WTBZ]均＜0.05）。结论宫缩乏力性产后出血产妇子宫平滑肌BKCa通道α、β亚基表达水平升高可能是发病的重要因素之一。

关键词: 产后出血, 宫缩乏力, 大电导钙激活钾通道

Abstract:

Abstract:Objective To detect the expression of large conductance Ca2+ activated K+ channel(BKCa) in myometrium in patients with postpartum hemorrhage(PPH).Methods The levels of contractile frequency, amplitude and contraction of myometrium were detected by RM6240 biological experimental system; the BKCa(α, β subunit) protein expression levels in myometrium were detected by Western blot; the BKCa(α, β subunit) mRNA expression levels in myometrium were detected by real-time fluorescent quantitative reverse transcription-polymerase chain reaction for women with PPH caused by uterine atony(study group, n=30) and normal uterine smooth muscle contraction(control group, n=30).Results (1) In study group, the levels of myometrium contractile frequency, amplitude and contraction were lower than that of the controls(P<0.05). (2) In study group, BKCa (α, β subunit) protein and mRNA expression in myometrium were significantly higher than that of control group(P<0.05). (3) Both groups, the level of myometrium contraction was negatively related to BKCa (α, β subunit) protein, BKCa (α, β subunit) mRNA level in myometrium.Conclusion Increased BKCa expression in myometrium may be involved in the pathogenesis of PPH caused by uterine atony.

Key words: postpartum hemorrhage, uterine atony, large conductance Ca2+ activated K+ channel

中图分类号:

R714.46+1

颜建英，廖秋萍，刘青闽，徐榕莉，黄晓燕，林顺和，陈文祯，罗美瑜. 子宫平滑肌大电导钙激活钾通道α、β 亚基表达与产后出血的关系[J]. 中国实用妇科与产科杂志.

YAN Jian-ying, LIAO Qiu-ping, LIU Qing-min, XU Rong-li,HUANG Xiao-yan,LIN Shun-he,CHEN Wen-zhen,LUO Mei-yu. . Expression of large conductance Ca2+ activated K+ channel in postpartum hemorrhage. [J]. Acta Metallurgica Sinica.

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