中国实用妇科与产科杂志

• 研究与探索 • 上一篇    下一篇

子宫平滑肌大电导钙激活钾通道α、β 亚基表达与产后出血的关系

颜建英廖秋萍刘青闽徐榕莉黄晓燕林顺和陈文祯罗美瑜   

  1. 作者单位:福建医科大学福建省妇幼保健院教学医院,福建 福州350001
  • 出版日期:2014-02-02 发布日期:2014-01-23
  • 通讯作者: 颜建英
  • 基金资助:

    福建省科技计划重点项目(2009Y0008);福建省医学创新课题(2009-CXB-32)

Expression  of large conductance Ca2+ activated K+ channel in postpartum hemorrhage. 

YAN Jian-ying, LIAO Qiu-ping, LIU Qing-min, XU Rong-li,HUANG Xiao-yan,LIN Shun-he,CHEN   Wen-zhen,LUO Mei-yu.    

  1. Department of Gynecology and Obstetrics, the Teaching Hospital Fujian Maternity and Children Health Hospital,Fujian Medical University,Fuzhou 350001, China
  • Online:2014-02-02 Published:2014-01-23

摘要:

目的 探讨产妇子宫平滑肌大电导钙激活钾通道(BKCa)α、β亚基表达变化在宫缩乏力性产后出血发病中的作用。方法 2009年10月至2011年2月福建省妇幼保健院产科,采用肌条等长张力实验测定30例宫缩乏力性产后出血产妇(病例组)和30例宫缩正常无产后出血产妇(对照组)子宫平滑肌收缩频率、幅度及收缩活动力;应用蛋白印迹法(Western blot)测定两组产妇子宫平滑肌BKCa通道α、β亚基蛋白表达水平;应用实时荧光定量聚合酶链反应(real-time RT-PCR)测定两组产妇子宫平滑肌BKCa通道α、β亚基mRNA表达水平。结果 (1)病例组子宫平滑肌自发宫缩收缩频率、幅度、收缩活动力均低于对照组,分别比较差异均有统计学意义(P<0.05)。(2)病例组子宫平滑肌BKCa通道α、β亚基蛋白表达水平及其mRNA表达水平均高于对照组,分别比较,差异均有统计学意义(P<0.05)。(3)两组产妇子宫平滑肌收缩活动力与BKCa通道α、β亚基蛋白表达水平及BKCa通道α、β亚基mRNA表达水平均呈明显负相关(病例组:r分别为-0.401、-0.727及-0.723、-0.895;对照组:r分别为-0.541、-0.378及-0.703、-0.625;[WTBX]P[WTBZ]均<0.05)。结论 宫缩乏力性产后出血产妇子宫平滑肌BKCa通道α、β亚基表达水平升高可能是发病的重要因素之一。

关键词: 产后出血, 宫缩乏力, 大电导钙激活钾通道

Abstract:

Abstract:Objective To detect the expression of large conductance Ca2+ activated K+ channel(BKCa) in myometrium in patients with postpartum hemorrhage(PPH).Methods The levels of contractile frequency, amplitude and contraction of myometrium were detected by RM6240 biological experimental system; the BKCa(α, β subunit) protein expression levels in myometrium were detected by Western blot; the BKCa(α, β subunit) mRNA expression levels in myometrium were detected by real-time fluorescent quantitative reverse transcription-polymerase chain reaction for women with PPH caused by uterine atony(study group, n=30) and normal uterine smooth muscle contraction(control group, n=30).Results (1) In study group, the levels of myometrium contractile frequency, amplitude and contraction were lower than that of the controls(P<0.05). (2) In study group, BKCa (α, β subunit) protein and mRNA expression in myometrium were significantly higher than that of control group(P<0.05). (3) Both groups, the level of myometrium contraction was negatively related to BKCa (α, β subunit) protein, BKCa (α, β subunit) mRNA level in myometrium.Conclusion Increased BKCa expression in myometrium may be involved in the pathogenesis of PPH caused by uterine atony.

Key words: postpartum hemorrhage, uterine atony, large conductance Ca2+ activated K+ channel

中图分类号: