中国实用妇科与产科杂志

• 研究与创新 • 上一篇    

银杏黄酮对人卵巢恶性畸胎瘤细胞株PA-1及人卵巢颗粒细胞的影响

杜辉,张琰,王惠兰,郝红娟,徐春琳   

  1. 作者单位:河北医科大学第二医院妇产科,河北 石家庄050000
  • 出版日期:2013-08-02 发布日期:2013-07-23

  • Online:2013-08-02 Published:2013-07-23

摘要:

目的 探讨银杏黄酮对人卵巢恶性畸胎瘤细胞株PA-1及人卵巢颗粒细胞体外增殖的影响。方法 采用噻唑蓝[3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di- phenytetrazoliumromide,MTT]法观察质量浓度分别为0、175、200、225 mg/L的银杏黄酮作用于PA-1细胞24、48、72 h后对细胞生长的影响;流式细胞仪检测作用48 h后对PA-1细胞凋亡率及细胞生长周期的影响;选取2011年3月至2012年9月河北医科大学第二医院生殖中心行体外受精-胚胎移植(in vitro fertilization and embryo transfer ,IVF-ET)患者的人卵巢颗粒细胞,将同等剂量的银杏黄酮作用于人卵巢颗粒细胞,经MTT法观察其对颗粒细胞的影响、经化学发光法检测颗粒细胞所分泌的雌二醇(E2)值的变化。结果 随着银杏黄酮浓度增高,对PA-1细胞的增殖有明显的抑制作用,呈明显时间、剂量依赖性;凋亡率随剂量增加而增加,细胞周期被阻滞于G1期;而同等剂量的银杏黄酮作用于人卵巢颗粒细胞后对其生长没有抑制作用,且各组的E2值差异没有统计学意义(P<0 .05)。结论 银杏黄酮明显抑制人卵巢恶性畸胎瘤PA-1细胞生长,而对卵巢的功能细胞-颗粒细胞没有损伤。

关键词: 银杏黄酮, PA-1, 细胞凋亡, 细胞周期, 颗粒细胞, 雌二醇

Abstract:

Abstract:Objective To investigate the impact of ginkgetin on the proliferation of PA-1 cell line of human ovarian malignant teratoma and human granular cell in vitro.Methods In the first place, PA-1 cells were treated with ginkgetin in different concentrations(0,175,200,225 mg/L) and cell proliferation of PA-1 was evaluated by MTT assay, the dose-effect relationship and the time-effect relationship were found. Cell apoptosis and distribution of cell cycle of PA-1 were evaluated by flow cytometry. In the next place, Human granular cells were treated with different concentrations(0,175,200,225 mg/L),the inhibit rates were evaluated by MTT assay, the level of E2 was assayed by chemiluminescence method.Results The inhibition rates of PA-1 cell increased according to the concentration of ginkgetin and time. The result of flow cytometry showed that ginkgetin could induce apoptosis of PA-1 cells and stop the growth of them at G1 period.Conclusion Ginkgetin can inhibit the growth of PA-1 cells apparently in vitro, and nearly has no effect on human granular cells. It is suggested that ginkgetin has some value in ovarian tumor and maybe bring no injury to normal human granular cells.

Key words: ginkgetin, PA-1, cell apoptosis, cell cycle, granular cell, E2

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