关键词: 巨噬细胞炎性蛋白-1α, 单核细胞趋化蛋白-1, 脂联素, 肥胖, 胰岛素抵抗

Abstract: Objective　To investigate the correlations between plasma monocyte /macrophage chemoattractant protein and related parameters of insulin resisitance in children with simple obesity. Methods　70 children with simple obesity were enrolled as study group，30 healthy children as control group. Body mass index（BMI ）and waist circumference （WC）were detected as indices of obesity .Plasma levels of macrophage inflammatory protein-1α（MIP-1α）、monocyte chemoattractant protein-1（MCP-1 ）were measured by ELISA.The rate of CD68 positive cells in blood were detected by flow cytometry（FCM）： Plasma levels of insulin 、adiponectin were measured by RIA， Insulin resisitance index （InRI） were caculated by homeostasis model assessment of insulin resistance （HOMA-IR）. The associations between MIP-1α、MCP-1 levels and adiponectin and related parameters were analyzed with Pearson correlation analysis or partial correlation analysis．Results　The concentration of MIP-1α and MCP-1 in plasma in children with simple obesity were higher than that in control group. There was a significant difference between the two groups （P < 0. 05）. The rate of CD68 positive cells in blood of simple obesity had no significant difference between that in control group （P > 0.05）.The concentration of MIP-1α and MCP-1 in plasma had positive correlations with BMI and WC and InRI（P < 0. 05）. The concentration of adiponectin in plasma had negetive correlations with BMI and WC and InRI （P < 0. 05）. The rate of CD68+ cell in blood had no correlation with BMI and WC and InRI（P < 0. 05）.Conclusion　We found that a differential low-grade inflammation is associated with obesity of children .The levels of MIP-1α、MCP-1 in blood of obesity were associated with insulin resistance and abdominal obesity. The levels of plasma MIP-1α、MCP-1、adiponectin may predict the onset of obesity related complications.

Key words: obesity, insulin resisitance , MCP-1, adiponectin