关键词: 血尿, 肾组织活检, 红细胞形态, 光学显微镜, UF50全自动尿液分析仪

Abstract: AbstractObjectiveThis study aims at evaluating the effect and the possible role of light microscopy and urine flow cytometer in detecting the source of hematuria in children,for a better understanding of various histopathology of isolated hematuria. Methods (1) The children who had been diagnosed as having glomerular diseases underwent renal biopsy.(2) The morning urine specimen from the group of glomerular diseases as well as the group of nonglomerular diseases were collected for the red blood cell morphological examination by light microscopy.In addition,the samples from some of the patients with glomerular diseases and some of the patients with nonglomerular diseases were examined by using UF50 urine flow cytometer. Results(1) The group of 125 children with clinical diagnosis of glomerular diseases were confirmed by pathological examination.(2) The diagnostic criteria for glomerular hematuria were those with severe dysmorphic RBC≥30% or dysmorphic RBC≥70%.Both specificity reached over 90%,but the sensitivity of the UF50 automated urine analyzer was lower.(3) The positive rate of glomerular hematuria by ordinary light microscopy and urine flow cytometer in testing the same day’s morning urine and random urine samples had no significant differences. Conclusion(1) This series of data indicate that if we use the diagnostic criteria for glomerular hematuria with dysmorphic RBC≥70% or severe dysmorphic RBC≥30%,the sensitivity and specificity are both higher.(2) Both specificity of the two methods reachs over 90%,but the sensitivity of the UF 50 automated urine flow cytometer is lower.(3)The random urine specimen could be accepted with the two methods in children.

Key words: Light microscopy, Sysmex UF 50 automated urine flow cytometer , Renal biopsy, Red cell morphology