关键词: 间隔缺损, 房, 猪, 相容性, 内皮化, 封堵装置

Abstract: AbstractObjectiveTo assess the biocompatibility and endothelialization of the homemade atrial septal defect occluder device. MethodsSix piglets were selected.Atrial septal defects with different diameters were created using the Brockenbrough needle and Rashkind balloon atrial septostomy.With the guidance of echocardiography and fluoroscopy,the propersized occluders were implanted corresponding to the stretched diameter of ASD measured by balloon.Two swine had been killed at 1 month,3 months and 6 months after transcatheter procedure.Macroscopic,microscopic assessment and scanning electron microscope (SEM) were done on the explanted devices. ResultsMacroscopic examination showed no excrescence,thrombus formation,arm fractures and corrosion.The devices were covered with collagen fibrosis and discrete endocardial cells,apparent inflammatory infiltration in the devices and around the devices 1 month after implantation.The implants were nearly endothelialized,while the inflammatory reaction relieved gradually,with myocardial cells ingrowth at the edges of the device 3 months after implantation.The devices were completely covered with endocardium and fibrous tissue.Moreover,endothelial cells could be found on the smooth microscrew adaptor.The inflammatory reaction diminished with a few chronic inflammatory cells existing.Neovascularization and lymphatic vessels ingrowth could be observed 6 months after implantation. ConclusionThe homemade atrial septal defect occluder device has good biocompatibility,nearly endothelialized at 3 months,completely by 6 months.

Key words: Endothelialization, Occlude device , Piglet , Biocompatibility