中国实用儿科杂志

中国实用儿科杂志

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儿童幽门螺杆菌空泡毒素基因的研究

唐清a，王琳琳a，梁淡湄a，姜海行b，陆红a   

  1. 广西医科大学一附院，a儿科，b消化内科；广西南宁530021
  • 收稿日期:2006-01-15 修回日期:2006-06-20 出版日期:2006-09-06 发布日期:2006-09-06

The research of Helicobacter pylori vacA genotypes in children.

Tang Qing,Wang Linling,Liang Danmei,et al.   

  1. Department of Pediatrics,the First Hospital Affiliated to Guangxi Medical University,Nanning 530021,China
  • Received:2006-01-15 Revised:2006-06-20 Online:2006-09-06 Published:2006-09-06

摘要： 目的探讨儿童幽门螺杆菌vacA基因型并分析各基因亚型与胃十二指肠疾病的关系。 方法对80例H·pylori感染的儿童，采用PCR法扩增胃黏膜vacA基因亚型。 结果南宁儿童H·pylorivacA基因型有s1a/m1和s1a/m2两种组合，基因频率分别为3.75%、66.25%。vacA基因各亚型在慢性浅表性胃炎及消化性溃疡中的检出率差异无显著性(P>0.05)。 结论s1a/m2为南宁地区儿童幽门螺杆菌的vacA优势基因型。部分患儿同时感染多株不同vacA基因型H·pylori。vacA基因各亚型不能作为南宁地区儿童H·pylori菌株毒力强弱的指标。

关键词: 幽门螺杆菌, 空泡毒素基因, 儿童

Abstract: AbstractObjectiveTo investigate the distribution of H·pylori vacA genetypes and their association with gastric duodenal diseases in children. MethodsEighty biopsy samples from patients with H·pylori infection were studied.PCR analysis for vacA genotypes was performed by using DNA extracted from biopsy specimens. ResultsIn 80 cases,there were two vacA mosaicisms including 3 for s1a/m1(3.75%) and 55 for s1a/m2(66.25%).No statistical differences were found in the distribution of vacA genotype of H·pylori strain infection between chronic superficial gastritis and peptic ulcer(P>0.05). ConclusionIn Nanning,vacAs1a/m2 genotype of H·pylori is predominant in children.Part of patients may be coinfected with different vacA genotypes of H·pylori.The vacA genotype can not be considered the markers of H·pylori which is more pathogenic in children in Nanning.

Key words: Children , vacA gene