关键词: 异物, 支气管肺泡灌洗液, 基质金属蛋白酶9, 羟脯氨酸, 基质金属蛋白酶抑制剂1

Abstract: To observe the extracellular matrix remodeling on children with airway foreign body aspiration (FBA) through detecting the expression of matrix metalloproteinase-9 (MMP-9),tissue inhibitor of matrix metalloproteinase-1 (TIMP-1),and hydroxyproline (HYP) in bronchoalveolar lavage fluid (BALF). Methods12 controls and 46 children with FBA removed via fiberoptic bronchoscopy from 2002~2003 were studied in our unit.Patients were divided into 3 subgroups according to the duration of FBA (subgroup A:<7 days; subgroup B:7~30 days; subgroup C:>30 days).Cell count,levels of MMP-9,TIMP-1 in BALF cells and HYP of BALF was measured. ResultsThe total number of cells and differential cells recovered in BALF did not differ significantly between patients and controls (P=0.431).The positive rate of MMP-9 in controls and subgroups of patients were 33.3%,62.5%,80.0%,and 93.3% with a significant difference (P=0.006).The positive rate of TIMP-1 was 16.3%,50.0%,80.0%,and 93.3% with a significant difference (P<0.001).The differences of HYP in controls and different patients are significant as well (P<0.001).There were positive correlations between the MMP-9,TIMP-1,HYP levels in patients and duration of FB retention (P<0.05).The levels of MMP-9 and TIMP-1 were also associated with percent of mast cell (P=0.009,P=0.005).The differences of MMP-9 and TIMP-1 before and after FB removed were not significant (P=0.131,P=0.522),while the HYP levels decreased significantly than that before FB removed (P=0.001). ConclusionMMP-9,TIMP-1 and HYP are significantly elevated in children with FBA and are related to the extracellular matrix remodeling.These changes are irreversible in short time

Key words: Hydroxyproline, Tissue inhibitor of matrix metalloproteinase-1 , Bronchoalveolar lavage fluid, Matrix metalloproteinase-9