中国实用儿科杂志

中国实用儿科杂志

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原发性肾病综合征患儿肾组织核因子-κB活化水平与病理类型及尿蛋白关系

赵红洋1;孙若鹏1;甄军晖2;董俊华1   

  1. 1山东大学齐鲁医院小儿内科(250012);2山东大学齐鲁医院病理科(250012)
  • 收稿日期:2004-12-12 修回日期:2005-03-10 出版日期:2005-06-25 发布日期:2005-06-25
  • 通讯作者: 赵红洋

The activation of nuclear factorKappa B in kidney tissue in children with primary nephrotic syndrome and its relationship with pathological changes and urine protein excretion.

Zhao Hongyang,Sun Ruopeng,Zhen Junhui et al.   

  1. Department of Pediatrics,Qilu Hospital of Shandong University,Jinan 250012,China
  • Received:2004-12-12 Revised:2005-03-10 Online:2005-06-25 Published:2005-06-25
  • Contact: Zhao Hongyang

摘要： 目的 探讨原发性肾病综合征(PNS)患儿肾组织核因子-κB(NF-κB)活化水平及其与肾组织病理类型、24h尿蛋白定量的关系。方法 2002年2月至2004年9月，收集山东大学齐鲁医院小儿内科51例PNS患儿活检肾组织及5例肾脏手术切除的正常肾组织。采用免疫组织化学染色法及多媒体彩色病理图文分析系统MPIAS-500，观察PNS患儿各病理类型肾组织及正常肾组织NF-κB的活化水平，同期收集PNS患儿24h尿蛋白定量。结果 微小病变型(MCD)、非微小病变型(NMCD)PNS患儿肾小球及肾小管中NF-κB活化水平均较对照组显著增强；NMCD患儿肾小球及肾小管中NF-κB的表达水平显著高于MCD患儿；20例系膜增殖性肾小球肾炎(MsPGN)患儿肾组织NF-κB活化水平随系膜增殖程度加重而增高；PNS患儿肾小球及肾小管NF-κB表达水平与24h尿蛋白定量呈显著正相关。结论PNS患儿肾小球及肾小管中NF-κB活化水平上调，且与组织病理类型、尿蛋白量相关，NF-κB的异常活化在PNS发病中起重要作用。


Abstract Objective To study activation of transcription factor NFKappa B(NF-κB) and its role in regulating the expression of inflammatory mediators involved in pathogenesis of primary nephrotic syndrome (PNS).Methods To explore the role of NF-κB in the pathogenesis of PNS in children,and its significance for pathological changes as well as 24hour urine protein excretion,we examined NF-κB activity in glomeruli and tubular tissues by twostep immunohistochemical staining from the kidney biopsy specimen of 51 children with PNS,and from 5 patients after renal operation.Results We found that NF-κB activity significantly increased both in MCD and NMCD children compared with the controls,and the activation of NF-κB in MCD was different from that of NMCD;there was also statistical difference of NF-κB activity between mild,moderate,severe MsPGN and the controls Additionally,positive correlation was observed between NF-κB activity of glomeruli and renal tubules and 24hour urine protein excretion.Conclusion These results suggested that the upregulated activation of NF-κB in glomerular and tubular tissues of children with PNS is related to severity,pathological changes and urine protein excretion,and involved in the pathogenesis of PNS.
Key words Nuclear factor kappa B;Primary nephrotic syndrome;Pathology;Children

关键词: 核因子κB, 肾病综合征, 病理学, 儿童

Abstract: Abstract Objective To study activation of transcription factor NFKappa B(NF-κB) and its role in regulating the expression of inflammatory mediators involved in pathogenesis of primary nephrotic syndrome (PNS).Methods To explore the role of NF-κB in the pathogenesis of PNS in children,and its significance for pathological changes as well as 24hour urine protein excretion,we examined NF-κB activity in glomeruli and tubular tissues by twostep immunohistochemical staining from the kidney biopsy specimen of 51 children with PNS,and from 5 patients after renal operation.Results We found that NF-κB activity significantly increased both in MCD and NMCD children compared with the controls,and the activation of NF-κB in MCD was different from that of NMCD;there was also statistical difference of NF-κB activity between mild,moderate,severe MsPGN and the controls Additionally,positive correlation was observed between NF-κB activity of glomeruli and renal tubules and 24hour urine protein excretion.Conclusion These results suggested that the upregulated activation of NF-κB in glomerular and tubular tissues of children with PNS is related to severity,pathological changes and urine protein excretion,and involved in the pathogenesis of PNS.

Key words: Children , Primary nephrotic syndrome, Pathology