Acta Metallurgica Sinica

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Study of proteins induced by telomere dysfunction in human IgA nephrology

WANG Min-minHE Qiang   

  1. Department of Kidney Disease,Zhejiang Provincial People’s Hospital,Hangzhou 310000,China
  • Online:2014-09-01 Published:2014-09-05

端粒缺陷相关蛋白在IgA肾病中的表达研究

王敏敏何强   

  1. 作者单位:浙江省人民医院肾脏病科,杭州 310000
  • 通讯作者: 何强
  • 基金资助:

    国家自然科学基金资助项目(81170691)

Abstract:

Abstract:Objective To observe the expression changes of telomere dysfunction related aging markers (CRAMP,stathmin,EF-1α,and chitinase) in the progression of IgA nephropathy,and to assess the prognostic value of these markers in predicting the renal injury degree during IgA nephropathy.Methods A total of 177 patients with IgA nephropathy and 83 healthy controls were enrolled in our study.The patients with IgA nephropathy were divided into IgAN Ⅰ-Ⅱ group and IgAN Ⅲ-Ⅴ group according to Lee’s grading system.Blood and urine samples were collected,and direct enzyme-linked immunosorbent assay (ILISA) was used to examine the expression levels of CRAMP,stathmin,EF-1α,and chitinase.Results (1)The expression levels of plasma CRAMP and chitinase were increased during the progression of human IgA nephropathy.Moreover,a combination of these two proteins could distinguish patients with IgA nephropathy from healthy individuals with a sensitivity of 88.2% and specificity of 92.5%.(2)The expression levels of urine CRAMP and chitinase were increased during the progression of human IgA nephropathy.A combination of these two proteins could distinguish patients with IgA nephropathy from healthy individuals with a sensitivity of 74.3% and specificity of 84.2%.Conclusion These data provide the evidence that telomere shortening related inflammatory proteins are associated with human IgA nephropathy,which may be a new direction for the disease progression study.

Key words: biomarkers, telomere, IgA nephropathy

摘要:

目的 观察IgA肾病患者血液、尿液中4种端粒缺陷相关蛋白CRAMP、stathmin、EF-1α和chitinase表达变化,评估其对IgA肾病肾损伤程度的预测价值。方法 以浙江省人民医院肾内科收治的177例IgA肾病患者为研究对象,同时选取83名健康对照者作为对照,其中IgA肾病患者根据肾穿刺活检病理分期分为IgANⅠ~Ⅱ组和IgAN Ⅲ~Ⅴ组。收集纳入者血浆及尿液标本,采用酶联免疫吸附法(ELISA)及Chitinase分析试剂盒检测CRAMP,EF-1α和stathmin浓度及chitinase酶活性。结果 (1)IgAN组血浆中CRAMP浓度及chitinase酶活性较对照组增高,且IgAN Ⅲ~Ⅴ组升高更为明显。联合CRAMP及chitinase对于区分IgA肾病和健康对照具有较高的灵敏度(88.2%)及特异度(92.5%)。(2)IgAN组尿液中CRAMP浓度及chitinase酶活性较对照组增高,IgAN Ⅲ+组chitinase酶活性升高更为明显。联合CRAMP及chitinase对于区分IgA肾病和健康对照具有较高的灵敏度(74.3%)及特异度(84.2%)。结论 端粒缺陷相关蛋白CRAMP及chitinase在IgA肾病表达增加,且二者对IgA肾病肾损伤程度具有较高的预测价值。

关键词: 生物标志, 端粒, IgA肾病

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