中国实用口腔科杂志

中国实用口腔科杂志

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冷常压等离子体处理对纯钛表面性能和成骨细胞增殖迁移的影响研究

谢艳婷1，李绍杰2，顾舒扬1，葛鑫1，田思睿1，赵宁宁1，江鹭鹭1，蔺增2，赵宝红1   

  1. 1. 中国医科大学口腔医学院·附属口腔医院种植中心，辽宁省口腔医学研究所口腔种植研究室，辽宁省口腔疾病重点实验室，辽宁省口腔疾病转化医学研究中心，辽宁  沈阳 110002；2. 东北大学机械工程与自动化学院，辽宁  沈阳  110004
  • 出版日期:2018-11-15 发布日期:2018-12-12
  • 基金资助:

    国家自然科学基金（51775096）；辽宁省自然科学基金（20170541059）；辽宁省科技计划项目（2013225049）

  • Online:2018-11-15 Published:2018-12-12

摘要：

目的　研究冷常压等离子体处理后纯钛表面性能变化及对成骨细胞增殖和迁移的影响。方法　本研究于2017年6月至2018年3月在中国医科大学附属口腔医院中心实验室完成。以冷常压等离子体处理后的纯钛片为实验组，未处理的纯钛片作为对照组。（1）通过X射线光电子能谱分析两组钛片表面的化学元素和价键状态，并应用静态接触角测量仪测量两组钛片表面的接触角。（2）通过MTT细胞增殖检测和划痕实验比较两组钛片表面接种的MC3T3-E1成骨细胞增殖和迁移速度。采用SPSS 17.0软件对数据进行统计学分析。结果　（1）实验组钛片表面TiO2和Ti-OH的相对含量百分比较对照组增加；实验组钛片表面的接触角为10.55° ± 0.94°，对照组钛片表面的接触角为46.68° ± 2.77°，两组差异有统计学意义（P < 0.001），且实验组钛片表面的接触角随着在空气中放置时间的延长而迅速增大，2 h后趋于稳定。（2）实验组钛片表面的MC3T3-E1成骨细胞增殖和迁移速度均明显较对照组快（P < 0.05）。结论　冷常压等离子体处理纯钛片表面后能增加其表面的亲水性，促进成骨细胞的增殖和迁移，可加快种植体植入后与骨组织的整合并促进骨组织的再生，具有重要的临床意义。但冷常压等离子体处理后的纯钛片表面亲水性会在空气中迅速降低，因此须将处理后的种植体立即植入种植窝洞中。

关键词: 冷常压等离子体, 接触角, 成骨细胞, 增殖, 迁移

Abstract:

Objective　To study the effects of cold atmospheric plasma on property of pure titanium surface and proliferation and migration of osteoblasts. Methods　This study was performed in Central Laboratory of Affiliated Stomatological Hospital of China Medical University from June 2017 to March 2018. The pure titanium treated with cold atmospheric plasma was taken as the experimental group and the untreated pure titanium as the control group. （1） X-ray photoelectron spectroscopy was used to analyze the chemical elements and valence bond states of the two groups of titanium plates，and the contact angle of the two groups of titanium plates was measured by the static contact angle measuring instrument. （2） The proliferation and migration of the osteoblasts were compared by MTT cell proliferation detection and scratch test. SPSS17.0 software was used to analyze the data statistically. Results　（1） The relative percentage of TiO2 and Ti-OH on the surface of pure titanium films increased after cold  atmospheric plasma treatment. The contact angle of the pure titanium plate after the cold atmospheric pressure plasma treatment was 10.55° ± 0.94°，and the contact angle of the control group was 46.68° ± 2.77°，and the difference between the two groups was statistically significant（P < 0.001）. Besides，the surface contact angle of the titanium plates of the experimental group increased rapidly with the exposure time in air，which was stable after 2 hours. （2） The cell proliferation and cell migration of MC3T3-E1 osteoblasts in the experimental group were significantly faster than in the control group（P < 0.05）. Conclusion　Cold atmospheric plasma on pure titanium surface can increase the hydrophilicity of pure titanium，promote osteoblast proliferation and migration，accelerate the osseointegration after implantation，and promote tissue regeneration. Thus，cold atmospheric plasma has important clinical significance. However，the hydrophilicity of the treated titanium plates decreases rapidly in the air，so the implants should be implanted into the sockets immediately after the cold atmospheric plasma treatment.

Key words: cold atmospheric plasma, contact angle, osteoblasts, proliferation, migration