中国实用口腔科杂志

中国实用口腔科杂志

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新型SD大鼠压迫性三叉神经痛动物模型建立及其作用研究

郭永峰，丁    纯，李明子，孙宁宁，朱冰洁，周    青   

  1. 中国医科大学口腔医学院口腔颌面外科，辽宁省口腔医学研究所，口腔颌面外科研究室，辽宁省口腔疾病转化医学研究中心，辽宁  沈阳  110002
  • 出版日期:2016-05-15 发布日期:2016-06-14
  • 基金资助:

    辽宁省自然科学基金项目（201202271）；沈阳市科学计划项目（F13-220-9-69）

  • Online:2016-05-15 Published:2016-06-14

摘要：

目的    本实验探讨建立一种新型SD大鼠压迫性三叉神经痛（TN）动物模型。方法    本研究于2014年6月于中国医科大学附属口腔医院中心实验室进行。雄性SD大鼠30只随机分为2组：实验组15只经脑定位仪3D定位至三叉神经根处，利用微量注射器将5%琼脂溶液10 μL注射到三叉神经根；对照组15只注射等量生理盐水。术前1 d和术后1、2、3、5、7、10、14、21、28、35、42 d分别进行行为学观察，Von Frey纤维测定大鼠机械性刺激反应阈值，利用方差分析及t检验对机械刺激阈值进行统计学分析。同时取压迫处三叉神经行苏木精—伊红（HE）及嗜银染色，并进行三叉神经节（TG）内星形胶质细胞胶质纤维酸性蛋白（GFAP）的免疫荧光检测。结果    实验组大鼠术侧机械刺激反应阈值在术后逐渐降低并稳定持续约30 d（P＜0.05），HE及嗜银染色显示神经纤维肿胀、脱髓鞘样改变；实验组大鼠术侧TG内星形胶质细胞GFAP表达明显增强。结论    琼脂压迫大鼠三叉神经根可以建立稳定的TN动物模型，为未来进一步研究TN病因及治疗提供一种可靠而有效的动物模型。

关键词: 三叉神经痛, 3D定位, 动物模型, 胶质纤维酸性蛋白

Abstract:

Objective    On the basis of this experiment in simulated clinical vascular compression of the trigeminal root，use the agar compression of trigeminal nerve root by 3D positioning to establish a new animal model of trigeminal neuralgia and relevant research. Methods    Adult male Sprague-Dawley rats were randomly divided into 2 groups：Experimental group，the rat head brain locator targeted to the trigeminal nerve root and 5% agar solution（10μL）was injected into the trigeminal nerve root by using miro syringe；Control group rats positioning micro syringe to the trigeminal nerve root injection of saline solution. Preoperative 1 d and postoperative 1，2，3，5，7，10，14，21，28，35，42 d respectively with behavioral observation of animals，Von Frey fiber mechanical stimulation reaction threshold determination in rats，use the ANOVA and T test for data statistics analysis. At the same time take the trigeminal nerve with HE and silver staining and trigeminal ganglion in the astrocytes GFAP immunefluorescence detection. Results    Experimental rat lateral mechanical stimulation reaction threshold operation gradually reduce and stabilize last about 30 days after surgery（P＜0.05）. HE and argentophilic staining showed swelling of the nerve fibers，demyelinating changes，the surgical operation side trigeminal ganglion of rats in astrocytes increased GFAP expression. Conclusion    Agar compression in the rat trigeminal nerve root can establish a reliable and effective animal model for the future further research of etiology and treatment of TN provide a reliable and effective animal model.

Key words: trigeminal neuralgia, TN；3D；animal model；glial fibrillary acidic protein, GFAP