关键词: 重组人骨形成蛋白-2, 胶原膜, 引导骨再生

Abstract:

Objective    To evaluate whether using a collagen membrane loaded with collagen-targeting recombinant human bone morphogenetic protein-2（rhBMP-2/CBD）would enhance bone regeneration in a rabbit guided bone regeneration （GBR） model. Methods    Four treatment modalities were randomly allocated on rabbit GBR model：rhBMP-2/CBD collagen membrane，rhBMP-2 collagen membrane，collagen membrane and no membrane. After two and six weeks，the rabbits were sacrificed. Hard tissue sections and paraffin sections were made and the new bones were examined by histological analysis. Results    At two weeks，the collagen membrane blocked the entry of fibrous tissue，and no new bone was observed in the superficial layer of the titanium cylinders in four groups. Howerver， capillary proliferation was significantly higher in group of rhBMP-2/CBD collagen membrane than in the other three groups. At six weeks，slender new bone trabeculae were observed in the superficial layer of the titanium cylinders in group of rhBMP-2/CBD collagen membrane，but not in the other groups. The boundary of the new bone from superficial layer or from the bone surface of the skull was obvious and higher degrees of bone were observed in group of rhBMP-2/CBD collagen membrane compared to the other three groups. Conclusion    Collagen membranes loaded with of rhBMP-2/CBD sustainedly release rhBMP-2 which induces new bone formation under the collagen membrane，improves in both quantity and quality in the superficial layer，and doubles the speed of new bone formation.

Key words: recombinant human bone morphogenetic protein 2, rhBMP-2；collagen membrane；guided bone regeneration, GBR