中国实用口腔科杂志

中国实用口腔科杂志

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miR-15b对口腔鳞癌细胞Cal27增殖与凋亡能力影响研究

郭    艳a，尚    超b，com/zgsykqk/CN/article/advancedSearchResult.do?searchSQL=(((任美思c[Author]) AND 1[Journal]) AND year[Order])" target="_blank">任美思c，com/zgsykqk/CN/article/advancedSearchResult.do?searchSQL=(((宋荣博a[Author]) AND 1[Journal]) AND year[Order])" target="_blank">宋荣博a，钟    鸣a，c   

  1. 中国医科大学a附属口腔医院 辽宁省口腔医学重点实验室，b基础医学院神经生物教研室，c附属口腔医院口腔组织病理学教研室，沈阳  110002
  • 出版日期:2015-08-15 发布日期:2015-09-09
  • 基金资助:

    国家自然科学基金（81301834）；辽宁省自然科学基金（2013021094）

  • Online:2015-08-15 Published:2015-09-09

摘要：

目的    研究口腔鳞癌（OSCC）中miR-15b的表达及其对OSCC Cal27细胞增殖和凋亡能力的影响。 方法    实时定量PCR检测2013年1月至2015年1月期间中国医科大学附属口腔医院外科50例住院患者OSCC组织中miR-15b基因的表达水平。培养OSCC Cal27细胞，将miR-15b的前体转染Cal27细胞，MTT法及双标流式法检测转染前后Cal27细胞的增殖和凋亡能力。 结果    与癌旁组织相比，miR-15b在OSCC组织中的表达显著下调，且与OSCC的分级相关。miR-15b的前体能够使Cal27细胞的增殖能力明显下降，且能够诱导Cal27细胞发生凋亡。结论    miR-15b的异常表达与OSCC相关，miR-15b能够抑制OSCC细胞增殖并诱导其凋亡，在OSCC中发挥肿瘤抑制基因的作用。

关键词: 口腔鳞癌, 增殖, 凋亡, miR-15b

Abstract:

Objective    To explore the expression of miR-15b in oral squamous cell carcinoma（OSCC） and the influence of miR-15b on OSCC Cal27 proliferation and apoptosis. Methods    Real-time PCR was used to detect the expression of miR-15b in OSCC and its adjacent tissues. The Cal27 cells were cultured and transfected with miR-15b precursor， then the proliferation and apoptosis ability of Cal27 cells were detected by MTT assay and double labeling flow cytometry method. Results    The miR-15b was down-regulated in OSCC tissues compared with the adjacent tissues and its expression showed a negative relationship with the grades of OSCC. The miR-15b precursor could significantly inhibit the Cal27 cells proliferation and induce the Cal27 cells apoptosis. Conclusion    The abnormal expression of miR-15b is involved in OSCC；miR-15b could inhibit the proliferation ability and induce apoptosis ability of Cal27 cells. The miR-15b acts as a tumor suppressor in OSCC.

Key words: oral squamous cell carcinoma, OSCC；proliferation；apoptosis；miR-15b