中国实用口腔科杂志

中国实用口腔科杂志

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犬牙槽骨牵张成骨牵张区新骨超微结构观察及钙磷元素含量分析

周苗1，车月娟2a，黄代营3，朴正国1，庾晓伟2b，陈松龄3   

  1. 作者单位：1.广州医科大学附属口腔医院，广州口腔疾病研究所·口腔医学重点实验室，广州510140；2.中山大学孙逸仙纪念医院a麻醉科，b口腔颌面外科，广州510120；3.中山大学附属第一医院口腔颌面外科，广州510160
  • 出版日期:2014-09-15 发布日期:2014-09-28
  • 通讯作者: 陈松龄
  • 基金资助:

    广东省科技计划重点资助项目（2004B33101010）；国际种植协会基金（847-2012）；国家自然科学基金项目（81000421）；广东省科技项目（2011B010200008）

Ultrastructural and Ca， P changes of the regenerated bone after alveolar distraction osteogenesis in canine model.

ZHOU Miao**， CHE Yue-juan ， HUANG Dai-ying， PIAO Zheng-guo ， YU Xiao-wei， CHEN Song-ling   

  1. *Key Laboratory of Oral Medicine， Guangzhou Institute of Oral Disease， Stomatology Hospital of Guangzhou Medical University， Guangzhou 510140， China
  • Online:2014-09-15 Published:2014-09-28

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摘要：

目的 探讨犬牙槽骨牵张成骨（alveolar distraction osteogenesis，ADO）牵张区新骨生长和改建的规律，为ADO技术在临床上应用提供实验依据。方法 本研究于2012年10月至2014年1月在中山大学附属第一医院实验动物中心完成。选择健康成年杂种犬12 只，拔除双侧下颌前磨牙，建立萎缩下颌牙槽骨动物模型。3个月后随机选择一侧萎缩下颌骨行牙槽骨切开术，植入2枚骨内型垂直向牙槽骨牵张器，经过7 d的间歇期，以每天1次，每次1 mm的频率进行垂直向增高，连续5 d。在固定期的第4、8和12周各处死4只犬并取材，采用扫描电镜/X-射线能谱联用仪观察牵张区新骨超微结构和测定其Ca、P元素含量。结果 扫描电镜观察可见牵张区新骨不断钙化的过程，0～4周为新骨形成早期，> 4～8周为中期，> 8～12周为后期；X-射线能谱分析显示了牵张区骨成熟的过程，Ca、P含量逐渐增高，12周时和宿主骨含量相当。结论 在牙槽骨牵张成骨的固定期，新骨逐渐形成并改建，至12周时成熟。

关键词: 牙槽骨牵张成骨, 扫描电镜, 能谱分析

Abstract:

Abstract：Objective To evaluate bone regeneration and remodeling of alveolar distraction osteogenesis （ADO），providing experiment evidence for the clinical application of ADO fechnique. Methods The experiment is performed in the Experimental Animal Center of the 1st Hospital of Sun Yat-sen University. Twelve healthy adult mongrel canines were provided， and they received posterior teeth extraction and alveoplasty in mandible to establish an atrophy alveolar model. After 3 months， a segmental alveolar osteotomy was made in the randomly selected unilateral atrophy alveolar and two alveolar distractors were placed. After a 7-day latency period， the alveolar ridge was augmented at a rate of 1.0 mm/d for 5 days. After a consolidation of 4， 8， and 12 weeks， the canines were sacrificed and the specimens of the distracted alveolar were harvested for scanning electron microscopic （SEM） study and element spectrum analysis of Ca， P contents. Results The distraction chamber revealed a distinctive dynamic ossification progress by SEM， early ossification stage in 1 to 4 weeks， intermediate stage of 4 to 8 weeks， and later remodeling stage of 8 to 12 weeks. Spectrum analysis indicated the maturation process of the alveolar distraction zone with gradient increase of Ca， P content，which was comparable to host bone at 12 weeks after distraction. Conclusion New bone is formed and gradually remodeled during the consolidation period of ADO， and mature at 12 weeks.

Key words: alveolar distraction osteogenesis, scanning electron microscopy, spectrum analysis

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