中国实用儿科杂志

中国实用儿科杂志

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UBE2C在神经母细胞瘤组织表达及临床意义分析

  

  1. 1.上海交通大学医学院附属新华医院 a小儿血液/肿瘤科；b病理科，上海  200092； 2.上海交通大学医学院附属上海儿童医学中心血液肿瘤科，上海  200127
  • 出版日期:2016-09-06 发布日期:2016-09-09

Expression and clinical significance of UBE2C in neuroblastoma

  1. Department of Pediatric Hematology/Oncology，Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine，Shanghai  200092，China
  • Online:2016-09-06 Published:2016-09-09

摘要：

目的    通过检测泛素偶联酶2C（UBE2C）在神经母细胞瘤（NB）患儿中的表达情况，分析其表达与NB患儿的临床特征及预后的相关性。方法    收集上海交通大学医学院附属新华医院2012年1月至2015年1月51例NB患儿术后石蜡包埋的组织样本，通过免疫组织化学方法检测其UBE2C 蛋白的表达水平。回顾性分析NB患儿的临床特征，采用Kaplan-Meier法分析其生存曲线。 结果    51例患儿中，男女比例为1.8∶1，中位诊断年龄为36个月（3.3～156.0个月），中位随访时间为25.6个月（5.5～42.7个月）。免疫组化结果显示，Ⅲ、Ⅳ期患儿UBE2C蛋白表达的阳性率（90.0%）明显高于Ⅰ、Ⅱ、Ⅳs期患儿（47.6%）（P＜0.001）；复发NB患儿的UBE2C表达阳性率（91.3%）明显高于无复发者（57.1%）（P＝0.001）。生存曲线分析显示， UBE2C蛋白阳性表达组预后较差（P＝0.006）。结论    UBE2C的表达与NB的分期及复发密切相关，UBE2C表达阳性提示NB患儿预后不良。UBE2C可能在NB侵袭、转移和复发过程中发挥重要的作用，有望成为NB潜在的生物标记物和治疗新靶点。

关键词: 神经母细胞瘤, UBE2C, 免疫组织化学, 预后

Abstract:

Objective    To detect the expression of UBE2C in neuroblastoma（NB） tissue and analyze its association with the clinical features of NB patients. Methods    Paraffin-embedded surgical tissue specimens from 51 NB patients kept by the Department of Pathology， Xinhua Hospital，from January 2012 to January 2015，were collected. The expression of UBE2C protein was detected by immunohistochemistry，and the clinicopathological characteristics of these patients were analyzed retrospectively. The survival curve was established with Kaplan-Merier analysis. Results    Of 51 cases of NB， the male-to-female ratio was 1.8∶1. The median age at diagnosis was 36 months（ranging 3.3-156 months） and median duration of follow-up was 25.6 months（ranging 5.5-42.7 months）. The results of immunohistochemistry showed that the UBE2C protein positive expression rate in stage Ⅲ and Ⅳ group （90.0%） was significantly higher than that in stage Ⅰ，Ⅱ and Ⅳs group （47.6%）（P＜0.001） ； the UBE2C protein positive expression rate in recurrence group（91.3%） was also higher than that in the non-recurrence group（57.1%）（P＝0.001）. The survival curve based on Kaplan-Merier analysis revealed that patients with UBE2C positive expression had poor prognosis（P＝0.006）. Conclusion    The expression of UBE2C protein is closely related to the stage and the relapse of NB patients. Positive expression of UBE2C protein implies the poor prognosis for NB. UBE2C may play an important role in the invasion，metastasis and relapse of NB. It might become a new biomarker and potential drug target to NB.

Key words: neuroblastoma, UBE2C, immunohistochemistry, prognosis